Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production

Thailand as an agricultural country faces significant challenges in managing the abundant biomass waste generated from activities. Conventional disposal methods such incineration contribute to pollution and limited availability of landfill space. To mitigate these issues valorization this has been a solution. This study focuses on utilization Napier grass renewable energy source. In experiment, samples were pretreated using oxalic acid with temperature variations (50 – 100 ° C), time (30 180 min), concentration (2 10%w/v) determine limit three factors for optimization studies. The Box-Behnken Design (BBD) within Response Surface Methodology (RSM) enabled determination optimal pretreatment conditions exploration correlation between reducing sugar content. model predicted 6% w/v, at C 105 min condition produce maximum 10.65 mg/ml. Therefore, sample was optimum results revealed amount obtained 10.67 mg/ml, which differed value error 0.22%. Thus, provides insight future researchers that can be applied pretreating maximize yield.